Abstract Endodontic infection is the infection of the root canal system and the microorganisms play a tremendous role in pulpal and periapical disease. The primary aim of endodontic treatment is to remove as many bacteria as possible from the root canal system. This can be achieved by a combination of local and systemic use of antibiotics. The use of antibiotics in dentistry especially in endodontics has become a practice towards inappropriate prescription and overdose leads to adverse side effects and bacterial resistance. An undergraduate student will have only limited knowledge in this regard. The aim of this study was to determine the knowledge, attitude and perception on usage of antibiotics among undergraduate students for endodontic therapy.This present study is a cross-sectional, descriptive questionnaire study conducted among dental undergraduate students in Chennai, India. The study was conducted from March to April 2020. A descriptive questionnaire study was conducted among 320 dental students in the city. A structured questionnaire was prepared focussing on antibiotics indication in dentistry particularly in endodontic treatment. Assessment of subjects awareness and attitude towards use of antibiotics for patients after endodontics procedures included in the questions relating to the knowledge of the basic antibiotics regime and its course. The subjects were requested to respond to each item in the specific format given. All the participants were allowed to choose one of the given three to four choices for each item in the questionnaire.A large population of students of 320 were familiar with this antibiotic resistance. Compared with the juniors, the senior students have a wider knowledge for prescribing antibiotics for various endodontic procedures.Most of the students prescribed Amoxicillin (30.82%)for any endodontic treatment for a duration of three days (26.42%) compared to other antibiotics.This study concludes that undergraduate dental students may prescribe antibiotics inappropriately and hence a proper and extensive guideline must be given to them to be followed . A thorough knowledge and awareness must be there on the antibiotic regime and its usage to all students before entering their clinics.