The present investigation aims to evaluate anticancer activity of methonolic extract of Solanum xanthocarpum fruits. The extract is investigated for its inhibitory effect on MCF7, HeLa, A549, CaCo2 cell lines. Percentage viabilities of cell lines are assessed by adopting the MTT method. The extract has significant cytotoxicity on MCF 7, HeLa, A549, CaCo2 cell lines in the concentration range between 10 to 100 µg/ml. as per MTT assay. IC50 values of Solanum xanthocarpum on MCF7, HeLa, A549, CaCo2 cell lines are 27.99, 75.55, 54.66 and 156.36 respectively. From the performed assay, methanolic extract has more cytotoxic effect on MCF 7 and least activity on CaCo2 cell line. Thus the extract of Solanum xanthocarpum fruits has identified to have anticancer activity