The present study was aimed to evaluate the free radical scavenging activity of hispolon and hispolon mono methyl ether (HMME) using different model systems such as nitric oxide and DPPH free radical scavenging activities; Ferric reducing antioxidant power (FRAP) and ABTS+ radical scavenging activity. Results of the study indicated that the inhibitory concentrations (IC 50) of hispolon (25.66), HMEE (24.84) and ascorbic acid (34.10)in nitric oxide free radical scavenging activity and hispolon (13.10), HMEE (26.92) and ascorbic acid (22.21) were observed in DPPH radical scavenging activity. The FRAP and TEAC values were also found to be significantly increased with hispolon and HMME in a concentration dependent manner compared with ascorbic acid. The antioxidant activity of hispolon and HMME might be due to electro donating capacity or hydrogen donating capacity to scavenge DPPH, nitric oxide and ferric ions.