Cleome viscosa Linn. (Capparidaceae), popularly known as “Asian spider flower”, is a native tree and found abundant in India. This study aimed to evaluate the antioxidant, anti-inflammatory, anti-allergic, and diuretic activity of the root extracts obtained from Cleome viscosa using in vitro and in vivo experimental models. Material and Methods: The ethanolic extracts and its fractions obtained from Cleome viscosa roots through Soxhlet extraction was analyzed by various activities. Different extracts were tested for total phenolic and flavonoid contents and in vitro antioxidant (ABTS & DPPH assay), anti-inflammatory (protein denaturation), anti-allergic (milk induced eosinophilia), and diuretic (Lipschitz test) activities. Results: Ethanolic extract exhibited the total phenolic (4.51±0.4) and flavonoid (5.34±0.93) content and highest antioxidant activity [{in ABTS (at 0.1 mg/mL; 33.62±0.76)} and {in DPPH (at 0.02 mg/mL; 29.30±0.56)} comparable to ascorbic acid, a standard compound {(0.04 mg/mL; 47.45±0.19) and (0.06 mg/mL; 97.14±0.53)} % inhibition] respectively. Whereas at the 150 µg/mL dose of EECV shows 51.07±0.92 % inhibition and viscocity 0.79±0.75 cp as compared to 625 µg/mL dose of Diclofenac sodium shows 56.23±1.98 % inhibition and viscocity 0.92±0.61 cp. Additionally, the total leucocyte count (at 100 mg/kg; 2483.34±148.14 per cu mm) and Eosinophils count (at 100 mg/kg; 66.6±10.54 per cu mm) was observed in anti-allergic activity when compared to the standard (Milk as 4 ml/kg; 3180.21±136.0 and 84.1±42.90) respectively. Also diuretic activity found significant in the petroleum ether fraction (200 mg/kg) as compared against the tested standard drug (Furosemide). Conclusions: It can be suggested that Cleome viscosa a great potential source of antioxidant, anti-inflammatory, anti-allergic and diuretic compounds useful for new diuretic drugs from the natural basis.