Abstract This study aimed determine the effect of Trichomoniasis on women who suffering from a delay in pregnancy despite previous normal pregnancy with vaginal infection, in order to prove the relationship of Trichomonas vagenalis to the obstruction of pregnancy in women, also detect of effect Trichomoniasis to a level of Gonadotropic Hormone:(Luteinizing Hormone LH and Follicle Stimulating Hormone FSH) Which are the most necessary hormones responsible for the composition and maturity of sexual reproduction cells, (200)sample was taken from infected women during the period ( September 2017 to October 2018), these samples examined by wet smear method for diagnostic Trichomonis vagenalis and recorded 51/200(25.5%) positive samples, also these samples examined using Real-time PCR on the basis of 18S rRNA gene and recorded (29.5%)59/200, this study also interested to detect of effect Trichomoniasis to a level of Gonadotropic Hormone:(Luteinizing Hormone LH and Follicle Stimulating Hormone FSH) by serological test, a results showed to a significant decrease in the level of hormones when recorded (17.313± 11.221) MUI / ml and (8.011 ± 7.001) MUI / ml respectively in patients, while their concentration was (21.623 ± 17..156) MUI / ml and (10.388 ± 9.835) in the control group.