Prevalence of ESBLs (blaSHV, blaTEM, blaCTX-M,) E.coli and K.pneumoniae from urinary samples. In this study, production of ESBL was measured in 48.8% (44/90) of K.pneumoniae that carried bla(SHV) (34/90, 37.7%), bla (TEM) (25/90, 27.7%), and bla (CTX-M) (35/90, 38.8%) and for 161 screening test positive E. coli, ESBL production was detected in 36.6% (59/161) of E. coli that carried bla(TEM) ,bla(CTX-M) and bla(SHV) genes in 5.5% (9/161), 28.57% (46/161) and 22.36% (36/161), respectively. Definitive identification of ESBL genes is only possible by molecular detection technique. Phenotypic tests need to be evaluated periodically as their performance may change with the introduction of new enzymes.