Introduction: Catheter-related bloodstream infection (CRBSI) is a critical problem in dialysis center. Aim: To study the prevalence of central venous catheter related blood stream infections and to identify the microbiological profile of organisms causing CRBSI. Materials and Methods: This prospective observational study carried out at Dialysis unit over a period of two years on 145 hemodialysis patients, who had cultures of catheter and blood samples were studied. Results: A total of 557 haemodialysis catheters (tunnelled and non-tunnelled) were inserted during the study period, and among them, CRBSIs was seen in 145 cases.Mean duration of dialysis catheters in situ was 20.6days.99 (68.2%) patients were males, and 46 (31.7%) were females. Diabetes mellitus (76.5 %) was the most common premorbid illness associated with CRBSI. 81.3% patients found to be below 10 mg/dl of Hb, and 68.3%patients had leucocytosis. Among 145 of CRBSI, blood cultures were positive in 48 patients, and 97 patients were culture negative.45.8% isolates were gram-positive and 52.8% isolates were gram-negative bacteria, and one isolate was found to be fungal infection.Among gram positive, CONS were most isolated, followed by Enterococcus, MSSA and MRSA. Among gram negative isolates, Acinetobacter were isolated in 5, Pseudomonas in 4, Klebsiella in 4,Citrobacter in 4, E. Coli in 3, Burkhel dorrhoea in 3 and Serratia in 2 cases respectively.The most sensitive routine antibiotic for P.aerusinosa isolated from CRBSI was piperacillin and ciprofloxacin(100%sensitive each). Among the reserved antibiotic, the most sensitive were cefepime (75%), cefoperazone-sulbactum, piperacillin-tazobactam and meropenem (100%) sensitive each. There was no routine antibiotic sensitive for E.coli isolated from CRBSI and three were ESBL producers and among reserved antibiotic meropenem was most sensitive. Conclusion: The knowledge of incidence of CRBSI and the microbiological spectra will be useful in formulating bundles of care and effective programs to control hospital acquired infections.