Introduction: Biofilm protects Enterococci from host immune response and antibiotics. Biofilm-producing Enterococci cause recurrent, chronic, and antibiotic-resistant infections. According to the National Institute of Health, 80% of infections are related to biofilm-forming microbes. Apart from biofilm-forming ability, Enterococcus spp. are known to produce various virulence factors. Biofilm formation is a major mechanism of adaptation that protects bacteria from antibiotics, due to several characteristics. Materials and Methods: This is a prospective, descriptive and observational study conducted in the Department of Microbiology, Index Medical College, Hospital and Research center Indore from January 2019 December 2021. All isolates of Enterococcus species during the study period will be included. Enterococci isolated from clinical specimens like pus, wound swab and aspirates etc. received in Microbiology Department. All samples were processed by standard bacteriological procedures. Gram staining was done for pus, wound swab and aspirates and findings were recorded. Culture was done on 5% sheep blood agar and MacConkey agar. Inoculated plates were incubated at 37°C for 18- 24 hours.