Abstract: The increasing number of epidemiological studies have consistently shown a protective effect against many diseases of polyphenol-rich foods (fruit, wine, chocolate...). The evidence is confirmed by the findings of various studies carried out in animal models, with nutritionally realistic amounts of isolated flavonoids, and with flavonoid-rich foods in humans. There have been huge advances in research on flavonoids due to their wide variety of pharmacological properties and a great devotion towards such flavonoids has gained much interest in preclinical research to explore them at molecular level. Natural bioactive compounds have wide and broad therapeutic actions that can be used for management of chronic diseases like Parkinson, Alzheimer, in this propose investigation centralizing on two such most promising bioactive flavonoids like, Andrographolide and Hesperidin and with the goal of identifying the potential synergistic impact adjacent to Scopolamine administration (1mg/kg, i.p.) for 7 days. There was a serious impairment in the behavior pattern (memory and learning), increase acetylcholinesterase enzyme activity, decrease oxidative status. The findings of the current investigation indicate that scopolamine intraperitoneal administration in mice causes neurobehavioral and biochemical changes that resemble those seen in Alzheimer's disease. Treatment of mice with a combination of AG and HSD is likely to have substantial neuroprotection mediated by its antioxidant properties and offers a good rationale for improved clinical control of Alzheimer's disease.