Background:Breast cancer continues to be a significant health threat, being the second most common cancer among Indian women. The present study was conducted to assess total PSA and free PSA levels in female patients with breast cancer. Materials & Methods: female patients with tumours growth of breast were included. Patients were classified into benign and malignant growth according to WHO classification. Patients were classified into group I (breast growth) and healthy subjects were put in group II. Parameters such as serum total prostate specific antigen (TPSA), serum free prostate specific antigen (FPSA) was recorded.