Abstract: This study examines the antiangiogenic effect of activated naked gold nanoparticles (AuNPS) in dorsal skinfold window chamber rat model after laser coagulation of dorsal blood vessels, to investigate whether the antiangiogenic effect of AuNPs can inhibit reperfusion and neovascularization of photocoagulated blood vessels in an animal model.This study includes 2 groups, each group contains 10 rats, laser group (control group), and AuNPs group. After laser photocoagulation of dorsal blood vessels through the dorsal window chamber fixed on the dorsum of albino rats, the structure of blood vessels and flow dynamics were documented with color digital photography to evaluate photocoagulation and reperfusion. The laser sessions and injection of AuNPs to the epidermal side of the window were twice weekly for 2 weeks. In the laser group (control group), 18 out of 20 photocoagulated blood vessels reperfused within 4-12 days with reperfusion rate 90%. In AuGNPs group 9 out of 22 photocoagulated blood vessels reperfused within 4-12 days with reperfusion rate 40%. Laser activated naked gold nanoparticles have significant antiangiogenic effect, with minimal side effects detected with local use.