Background: The laparoscopic cholecystectomy (LC) is the gold standard to treat gallstones. The present study was conducted to compare high- and low-pressure carbon dioxide pneumoperitoneum in patients undergoing laparoscopic cholecystectomy (LC). Materials & Methods: 80 patients undergoing laparoscopic cholecystectomy were divided into 2 groups of 40 each. In group I, the pneumoperitoneum with PaCO2 of 7– 10 mmHg and in group II with 12–14 mmHg using standard four-port method was used. Abdominal pain at the site of surgery and shoulder-tip pain were assessed based on the verbal rating scale (VRS). Parameters such as arterial blood pressure, heart rate, and body temperature were recorded during and 1 hour, 6 hours, 12 hours and 24 hours after the surgery